spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Sequestration of Cx43 within early endosomes in Cx33 transfected Sertoli cells. (A-T) Sertoli cells expressing Cx33 were transfected with EGFP-rab5, EGFP-rab7 or EGFP-lamp vectors or stained with antibodies specific of ER or Golgi apparatus. Fluorescent deconvolution microscopy analysis allowed the simultaneous detection of Cx33 (A,E,I,M,Q), Cx43 (B,F,J,N,R) with Endoplasmic Reticulum (C), Golgi apparatus (G), early endosomes (K), late endosomes (O) or lysosomes (S). (D,H,L,P,T) Merged images of Cx43, myc and EGFP localization. White spots indicate colocalization of the three signals. Note that the Cx33/Cx43 complex colocalizes mainly with EGFP-Rab5 (L) and weakly with ER (D). Results are representative of three separate experiments. Magnification, x800.





Right arrow Return to article