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Fig. 3. ES cells express active BMP4 and 7 but not BMP2. (A) RT-PCR on cDNA from wild type and transgenic ES cell lines BRE-luc, BRE-lac1 and BRE-lac2. All the lines tested express BMP4 and BMP7, but not BMP2. Using the same primer sets, transcripts for BMP2, 4 and 7 were detected by RT-PCR on cDNA from E9.5 mouse hearts, as expected (results not shown). ß-actin was a positive control for the reverse transcription reaction; expression of Oct4 indicates the undifferentiated state of the ES cells. (B) Transactivation assay in HepG2 cells. Medium conditioned by ES cells induces BRE-luc reporter activity in HepG2 cells. Non-conditioned medium had no effect. As a positive control, 10 ng/ml BMP4 was added to transfected cells to activate the BRE-luc reporter. As a negative control, HepG2 cells were transfected with the BRE-luc reporter and no BMP was added. Results are presented as mean fold induction (±s.d.) of triplicates in relation to unstimulated cells, set to 1 (^*P0.05).

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