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Fig. 2. Binding of {epsilon}-PKC to cardiac myofilaments. (A) Western blot of myofilament pellets showing binding of purified {epsilon}-PKC in absence or presence of 50 µM arachidonic acid (AA). {epsilon}-PKC concentrations (in µM): lane 1, 0; lane 2, 0.1; lane 3, 0.2; lane 4, 0.5; lane 5, 1. (B) Summary of {epsilon}-PKC binding to myofilaments. The solid circles (in the presence of AA) represents a fit to B/Bmax=[{epsilon}-PKC]/([{epsilon}-PKC] + EC50), with EC50=86 nM and Bmax=15 pmol (or 75 pmol/mg protein). The stoichiometry of binding was 15 pmols {epsilon}-PKC bound per 2 nmoles actin monomers or a 1/130 ratio. The open circles represent a linear regression of {epsilon}-PKC binding in the absence of AA.





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