Fig. 1. Cuticle morphology is disrupted in Df(1)MR adult wings. Wild-type and Df(1)MR wings dissected 2 hours after adult eclosion. (A-D) Light microscopy. The mutant wing in B is reduced in size, although veins (L2-L5) and wing margin (WM) are correctly patterned. (C,D) A closer view of the same wings reveals that hairs are much closer in the mutant than in the wildtype (arrowheads). (D) The picture of the mutant corresponds to a slightly lower focus plane, showing the cell outlines. Note the ring of cuticle surrounding each hair in the mutant. (E,F) SEM micrographs. The mutant hairs appear twisted and branched (open arrowheads), in contrast to the long and slender wild-type epidermal hairs (E). Some cuticle blobs appear bulging out of the main wing surface in the mutant (arrow). (G-J) TEM sections. The two wing surfaces (dorsal and ventral) are separated in the mutant (H,J), and the resulting space contains cuticle invaginations (small arrows) and cell debris (arrows). The presence of cuticle defects is indicated by arrowheads and at a higher magnification in the lower panels (I,J). The position of some epidermal hairs is also indicated (open arrowheads in G,H).

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