Fig. 6. Quantitative accumulation of externally added BSA-histone conjugates into Colo-205 cells. Bb-histone conjugates were prepared and their accumulation within the cell cytosol and nuclei was estimated as described in Materials and Methods. ATP depletion was performed as described in Table 1. , nuclei; , cytosol. The amount of Bb-histone present in the nuclei of cells incubated in 37°C was considered as 100% and represents 6.3 nmol/mg protein; it was estimated to be about 28% of the total added Bb-histone. The ratio given are in mole:mole.

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