Fig. 4. CD82 is associated with ErbB2 and ErbB3. (A) Expression of ErbB proteins in MCF-7/CD82, HB2/CD82 and T47D cells. Equal amounts of total protein lysates were resolved in 8% SDS-PAGE, transferred to the membrane and probed with antibodies to EGFR (mAb Ab-15), ErbB3 (C-17) or ErbB2 (Ab-1). (B) Cells were lysed in 1% Brij98 and the complexes were immunoprecipitated using specific antibodies: anti-ErbB3, mAb-4; anti-ErbB2, mAb-11; anti-CD82, C11; negative control, 187.1. The immunoprecipitated complexes were resolved in 8% SDS-PAGE, transferred to a nitrocellulose membrane and probed with Abs to ErbB3 (C-17) or ErbB2 (mAb-1) or CD82 (mAbs C33 and TS82b). (C) Stimulation with HRG does not affect the association of CD82 with ErbB2 and ErbB3. MCF-7/CD82 cells were stimulated with 50 ng/ml HRG for indicated times at 37°C. CD82-ErbB complexes were analysed as described above.

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