Fig. 9. The Bpag1 isoform 2 N-terminus, but not the isoform 1 N-terminus, localizes to the nucleus of C2C12 cells when the plakin domain is included in the fusion protein. (A-C) Nterm1long (A), similar to the shorter Nterm1 fusion protein (Fig. 6E), localized to actin aggregates (B; arrows in A and B). This protein was never observed in the nuclei (arrowhead in C). (D-F) Nterm2long (D) localized to the nucleus in almost all cells (arrowhead in D and F), and was also found in the cytoplasm in over half of the cells, including along stress fibers (E; arrows in D and E). (G,H) In many of the cells, Nterm2long staining (G) appeared to be exclusively nuclear, in a Swiss-cheese pattern of staining reminiscent of the endogenous Bpag1 staining (see Figs 3 and 5). (I) The C-terminal half of the plakin domain (plakinCterm) localizes in a similar pattern in the nucleus in all cells expressing this fusion protein. Additional localization to the cytoplasm occurred only in a minority of the cells expressing plakinCterm. Bars, 20 µm.

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