Fig. 6. The interaction of Bpag1 N-terminal fusion proteins with actin filaments in C2C12 myoblasts. (A-D) The FLAG-ABD protein (A) coaligned with actin stress fibers (B). A relatively stronger signal was also consistently noted at the ends of the fibers (insets in A-C; arrow in D). (E-H) The N-term1 protein (E) colocalized primarily with actin aggregates (F) in all cells. It never colocalized with normal-appearing stress fibers. (I-L) The Nterm2 fusion protein (I) co-aligned with stress fibers (J), but not at the ends (left-hand insets in I-K; arrows in L). In about half of the cells, Nterm2 colocalized with aberrantly bundled actin fibers (right-hand insets in I-K). Bar in K, 20 µm for all conventional fluorescence micrographs. Bars in confocal micrographs (D,H,L), 10 µm.

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