Fig. 2. Dynamics of the vault complex measured by FRAP. (A) The graph shows the mobility of fluorescent MVP-GFP (red line) compared with free GFP (green line) and a GFP-tagged truncated MVP that is not incorporated into vaults (MVP706-GFP, blue line), each measured in 12 cells. Fluorescence intensity before the bleach pulse was set at 1. Note that the recovery of fluorescence is never 100% because of removal of fluorescence by the bleach pulse. This effect differs between proteins depending on their residence time in the strip during bleaching. (B) The redistribution of fluorescent MVP-GFP within a bleached strip was monitored at 37°C (red line) and at 28°C (blue line) with intervals of 100 milliseconds. The graph depicts the data of an experiment in which 21 cells were measured.

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