Fig. 11. Recycling of Tfn in control, Syt IX-overexpressing and Syt IX-suppressed RBL cells. RBL-Syt IX^– (A,D,G), control (B,E,F) and RBL-Syt IX⁺ (C,F,I) cells were grown on glass coverslips, serum starved for 1 hour and incubated with TR-Tfn (50 µg/ml) for 1 hour at 37°C. Cells were subsequently placed on ice (A-C) or washed and subjected to a chase in the presence of unlabeled Tfn (100 µg/ml) and defroxamine mesylate (100 µM) for 30 minutes (D-F) or 1 hour (G-I). Cells were processed and visualized by confocal microscopy, as described under Materials and Methods. Bars, 13 µm.

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