Fig. 6. Effects of cytoskeleton disruption on long-range movements of WPb. (A) Trajectories of the ten most mobile WPb in a nontreated cell (NT), a nocodazole-treated cell (noco) and a latrunculin B-treated cell (latB). Bars, 10 µm. (B) Analysis of long-range motions in nontreated cells (NT, n=54 from three cells), nocodazole-treated cells (noco, n=2 from three cells), cells treated with the kinesin ATPase inhibitor ATA (n=16 from three cells), latrunculin B-treated cells (n=33 from three cells) and cells treated with the myosin ATPase inhibitor BDM (n=44 from three cells). Parameters derived from three-dimensional tracking are: frequency of long-range motions (in s^-1), defined as the number of WPb undergoing long-range motions per total number of WPb per unit time, average and maximum velocities (in µm/second) and total run length (in µm).

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