Journal of Cell Science 116, e1404 (2003)
Copyright © 2003 The Company of Biologists Limited
SETA adapts to adhesion
SETA (also known as CIN85 or Ruk) is an SH3-domain-containing adaptor
protein that functions in several signalling pathways – for example, it
regulates PI 3-kinase (PI3K) signalling by interacting with the PI3K
regulatory subunit p85
and facilitates internalization of receptor
tyrosine kinases by binding to the ubiquitin ligase Cbl. Oliver Bögler
and co-workers have found that SETA has another string to its bow: it's
involved in cell adhesion (see
p. 2845). Using
confocal microscopy, they observe that SETA co-localizes with actin along
microfilaments and at focal adhesions, as well as with microtubules. The
protein does not interact with cytoskeletal filaments directly; instead these
interactions are mediated by the SETA partner AIP1, which the authors show
binds strongly to actin and tubulin. Bögler and co-workers can also
identify SETA and AIP1 in complexes containing focal adhesion kinase (FAK) and
its relative PYK2. Moreover, they demonstrate that SETA and AIP1 can regulate
cell adhesion in electrical cell-substrate sensor (ECIS) attachment assays and
that AIP1 attenuates FAK/PYK2 phosphorylation. The authors thus conclude that
SETA and AIP1 can regulate cell adhesion, suggesting they do so as part of a
focal adhesion kinase regulatory complex.
Related articles in JCS:
- SETA/CIN85/Ruk and its binding partner AIP1 associate with diverse cytoskeletal elements, including FAKs, and modulate cell adhesion
- Mirko H. H. Schmidt, Baihua Chen, Lisa M. Randazzo, and Oliver Bögler
JCS 2003 116: 2845-2855.
[Abstract]
[Full Text]
