Fig. 10. Inhibition of myotube formation by EGFP-tagged murine archvillin sequences. EGFP-tagged proteins encoding the designated murine archvillin amino acids were transfected into C2C12 myoblasts, and the percentages of expressing cells that were multinucleate were scored after 6 days in differentiation medium. Column heights and error bars represent the means and standard deviations, respectively, of four separate experiments. Statistical significance was assessed with the Tukey-Kramer multiple comparisons test and by Student's t-tests. Both analyses indicated that C2C12 cells transfected with EGFP-tagged murine archvillin sequences 1-1353, 1-739 and 257-739 were significantly less likely to be present in myotubes than were cells similarly transfected with either the EGFP tag alone or with EGFP-tagged full-length murine archvillin (*P<0.001).

Return to article