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Fig. 6. Effect of hypoxia on RhoA and Cdc42 mRNA levels in renal cell carcinoma.
Total RNA was isolated from Caki-1 cells in normoxia and hypoxia. RT-PCR
amplification was performed using primers for RhoA for 15 to 30 cycles in
order to define optimal conditions (A). At 25 cycles, RT-PCR analysis for RhoA
(183 bp), Cdc42 (400 bp), and 
-tubulin (321 bp) were carried out on
total RNA isolated from cells incubated under hypoxia for up to three hours
(B).
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