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Fig. 3. Subcellular distribution of RhoA and Cdc42 proteins during hypoxia. After hypoxic treatment, renal cell carcinoma were fractionated into soluble and crude membrane fractions by centrifugation at 100,000 g for 1 hour. Each fraction (20 µg of protein) was separated by SDS-PAGE then immunodetected using Cdc42 (A) and RhoA antibodies (B). Densitometric analysis of both GTPases was carried out for soluble ({square}) and membrane ({blacksquare}) fractions. Means ± s.e.m. are shown from three independent experiments relative to samples from normoxic cells. Significant differences (P<0.05) from normoxia are indicated by asterisks (*).





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