Fig. 1. PP1 transiently associates with the NE. (A) AKAP149 was immunoprecipitated from NEs after release of HeLa cells from mitotic arrest. Dissociation of PP1 from immunoprecipitated AKAP149 was monitored by immunoblotting. AKAP149 was also immunoblotted using anti-pS antibodies. Apparent M_r is shown in kDa. (B) Entry into the cell cycle after release from mitotic arrest was monitored by BrdU incorporation from time of release (arrow). Relative BrdU labeling intensity in two replicates is shown (n=40 cells/time point). (C) 293T, HUVEC, Jurkat and Bjab cells in G1 or S phase (2 and 15 hours post-release from mitotic arrest, respectively) were immunoblotted using anti-PP1 antibodies. NEs were prepared from the G1- and S-phase cells and immunoblotted.

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