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Fig. 7. The extent of p368 phosphorylation is increased through the cell and is maximal during S and G2/M. Synchronized cells collected at the indicated cell-cycle stage were processed for immunoblotting and probed with antibodies to p368 ({alpha}-p368), Cx43NT1 ({alpha}-Cx43), or vinculin (for a loading control). The molecular weight or migration position of the Cx43 is indicated on the right, and the ratio of the extent of p368 to Cx43NT1 antibody labeling is shown on the bottom line.





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