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Fig. 6. Concentration dependence of LPA-induced LPA1 internalization. Stably transfected HeLa cells expressing LPA1 were incubated for 30 minutes with different concentrations of LPA, fixed and processed for indirect immunofluorescence localization of LPA1. Endosomal labeling of LPA1 can be observed even after treatment with 0.01 µM LPA. Bar, 10 µm.





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