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Fig. 5. Agonist removal stimulates the recycling of internalized LPA1 back to the PM. (A) Stably transfected HeLa cells expressing LPA1 were incubated in the absence (Untreated) or presence (+LPA) of 10 µM LPA for 30 minutes. (B) The cells were then rinsed, incubated in serum-free medium for the indicated times and processed for indirect immunofluorescence localization of LPA1. Bar, 10 µm.





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