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Fig. 2. LPA induces the time-dependent internalization of LPA1 in HeLa cells. Stably transfected HeLa cells expressing LPA1 were incubated for various times with 10 µM LPA, fixed and processed for indirect immunofluorescence localization of LPA1 using mouse anti-FLAG antibodies and Alexa594-labeled goat anti-mouse secondary antibodies. LPA1+ endosomal structures are first observed after 10 minutes of LPA treatment. Bar, 10 µm.





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