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Fig. 3. The mRNA levels of AJ and TJ constituents in epithelial cells overexpressing Snail. (A) Northern blotting of Eph4, Eph4-mSnail and NIH/3T3 cells. Snail completely shut down the transcription of E-cadherin, claudin genes and occludin, but not of ZO-1 or cytokeratin-18. As a control, the GAPDH gene was detected in equal amounts in all samples. (B) Comparison of the mRNA levels of E-cadherin, claudin-4, claudin-7, occludin, Snail and ZO-1 between mouse epithelial cells (Eph4, MTD-1A and CSG1) and fibroblasts (NIH/3T3 and L).





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