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Fig. 4. Coexpression of TACE and SAP97 alters their cellular distribution. COS-7 cells were transfected with different forms of haTACE and GFP-SAP97 and their cellular distribution was analyzed. (A) GFP-SAP97 presents a distribution similar to that of GFP-SAP97DPDZ3. (B) haTACE presents a distribution similar to that of haTACEm. (C) Distribution of GFP-SAP97 when coexpressed with haTACE (three representative cellular distributions are shown). (D) Distribution of GFP-SAP97 when coexpressed with haTACEm. (E) Distribution of GFP-SAP97DPDZ3 when coexpressed with haTACE. Distribution of GFP-SAP97 overexpressed forms were assessed by GFP epifluorescence with a FITC filter. TACE overexpressed forms were detected using anti-HA epitope antibodies followed by Rhodamine-conjugated secondary antibodies and their distributions revealed by Rhodamine epifluorescence.





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