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Fig. 5. Comparison of the distribution of amphiphysin 2 with markers of
intracellular organelles. HeLa cells were transiently transfected with Bramp2
in pRK5myc, pre-permeabilized in 0.01% saponin and fixed with PFA. Then, they
were stained for myc expression and organelle-specific markers: anti-EEA1
(early endosomes), anti-CD63 (late endosomes/lysosomes), anti-LAMP1
(lysosomes), and anti-calnexin (endoplasmic reticulum). In the case of
GFP-Rab11, cells were co-transfected with Bramp2 in pRK5myc and GFP-Rab11
before staining with anti-myc antibody. Images were acquired on a confocal
microscope and pseudo-colored with Metamorph. Colocalization appears in
yellow.
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