Fig. 7. Western blots for phosphorylated ERK (A) and total ERK (B) from SH-SH5Y cells not infected (Con) or infected with adenovirus to express green fluorescent protein (GFP) or constitutively active (CA) Rit, Ras or Raf, quantified in arbitrary units compared with uninfected cells (C), demonstrate that CA-Rit, CA-Ras, and CA-Raf all increased ERK phosphorylation. Western blots for phosphorylated Akt (D), and total Akt (E), quantified in F, demonstrate that CA-Ras, but not CA-Rit, increased Akt phosphorylation. Treatment of CA-Rit infected cells with the MEK inhibitor PD 098059 (Rit, PD) inhibits Rit-induced ERK phosphorylation (A,C) but not Akt phosphorylation (F). Data shown are representative blots from an experiment performed three times (quantification includes all three experiments).

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