Fig. 6. Quantification of neurite outgrowth from SH-SY5Y cells expressing CA-Rit, CA-Ras or CA-Raf. CA-Rit significantly increases the number of neurites per cell (A, including cells not bearing neurites), the percentage of neurite-bearing cells (B), the length of the longest neurite per cell (C) and the number of branch points per neurite at three days (open bars) or two weeks (filled bars) after initial infection. In contrast, CA-Ras or CA-Raf increase neurite initiation (the number of neurites per cell, A; the percent of neurite-bearing cells, B) or elongation (the total neurite length per cell, C) over two weeks (CA-Ras also increased the total neurite length per cell at three days, C). Results are means±s.e.m. of pooled data from three separate experiments. Asterisks (*) indicate significant difference compared with Control or GFP only cultures at P<0.05 (ANOVA with Least Significant Difference post hoc test).

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