Fig. 4. Quantification of the number of neurites per cell (A, excluding cells not bearing neurites), the percentage of neurite-bearing cells (B), the total neurite length per cell (C) and the number of branch points per neurite (D) from SH-SY5Y cells cultured on laminin-1. Both wild-type (WT-Rit) and constitutively active (CA-Rit) Rit increase neurite initiation (A,B), elongation (C) and branching (D, CA-Rit only) compared with control cultures not infected with adenovirus (Control) or cultures infected with adenovirus expressing only green fluorescent protein (GFP). Results are means±s.e.m. from four separate experiments. Asterisks (*) indicate significant difference compared with Control cultures at P<0.05 (ANOVA with Least Significant Difference post hoc test).

Return to article