Fig. 1. Rit supports neurite outgrowth in SH-SY5Y cells on endogenous matrix. SH-SY5Y cells were either not exposed to adenovirus or infected with adenovirus expressing only green fluorescent protein (GFP) or co-expressing GFP and wild-type (WT-Rit) or constitutively active Rit (CA-Rit). Expression of the transgene was confirmed by western blotting for Rit (A). Images of uninfected cells (B) or cells infected with GFP alone (C, and corresponding fluorescent image D) displayed basal levels of neurite outgrowth. Cells co-expressing GFP and either WT-Rit (E, and corresponding fluorescent image F) or CA-Rit (G, and corresponding fluorescent image H) displayed a qualitative increase in neurite outgrowth from SH-SY5Y cells. Bar, 50 µm.

Return to article