Fig. 8. Effect of expression of R2-PPP4c on the localisation pattern of newly formed snRNPs in HeLa cells. HeLa cells were injected with expression plasmid DNAs pEYFP-SmB, pEGFP-R2 and/or pCMV5-HA-PPP4c (each at 15 µg/ml), which are capable of expressing YFP-SmB, GFP-Flag-R2 (termed hereafter GFP-R2) and/or HA-PPP4c. 2 hours after injection cells were fixed in 3.7% paraformaldehyde. Images were collected on a Zeiss Delta Vision microscope and digitally deconvoluted. Panels A and J were visualised using anti-HA antibodies and secondary antibodies conjugated to Cy3. DNA was detected with DAPI. (A-D) HeLa cells injected with a mixture of plasmid DNAs expressing GFP-R2, HA-PPP4c and YFP-SmB. (E,F) HeLa cells injected with plasmid DNA expressing YFP-SmB alone. (G-I) HeLa cells injected with a mixture of plasmid DNAs expressing GFP-R2 and YFP-SmB. (J-L) HeLa cells injected with a mixture of plasmid DNAs expressing HA-PPP4c and YFP-SmB. Bar, 10 µm.

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