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Fig. 3. Analysis of Flag–R2-PPP4c material by gel filtration. Peak fractions from the anti-Flag agarose column containing Flag-R2 and PPP4c were pooled and subjected to Superose 6 gel filtration chromatography. Fractions eluted were analysed by SDS-PAGE and subsequent immunoblotting with anti-Flag (for detection of Flag-R2) and anti-PPP4c antibodies. Superose 6 fraction numbers are indicated above each lane. Molecular mass markers, thyroglobulin (670 kDa) and ferritin (450 kDa) are indicated by arrows.





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