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Fig. 1. Expression R2 and PPP4c mRNAs in human tissues. Human multiple tissue
northern blots (Clontech) were hybridised with a R2 cDNA probe (
0.8 kb,
coding for amino acids 78-316) and PPP4c cDNA probe (
0.8 kb, coding for
amino acids 12-279) and a ß-actin control probe. ß-actin mRNA was
used as a control to assess the variation in sample loading. Hybridisation
using each probe was carried out separately, and the blot was stripped before
being re-hybridised with the next probe. Transcripts are indicated by arrows
on the right, and the positions of RNA standards of known sizes are indicated
in kb on the left.