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Meiotic recombination-related proteins

Reciprocal genetic exchange between paternal and maternal alleles during meiosis occurs at synaptonemal-complex-associated recombination nodules (RNs). These are visible by EM and contain proteins such as the E. coli MutL homologue MLH1. In early prophase, each chromosome has 10 `early nodules' containing the RecA-type recombinases RAD51 and DMC1, but only one or two RNs are present by late prophase. How are most of the DNA-DNA interactions eliminated? Peter Moens and co-workers have approached the problem by integrating fluorescence microscopy, EM and immuno-EM to follow the dynamics of recombination-related proteins at these nodules in mice (see p. 1611). They demonstrate that the sites sequentially recruit biochemical activities: RAD51 and DMC1 arrive first, but are later replaced by replication protein A (RPA) and the Bloom helicase (BLM), and ultimately MLH1 (a protein essential for meiotic recombination) — interestingly, the MLH1 and BLM foci do not coincide. The findings suggest that RAD51 and DMC1 initiate meiotic DNA-DNA interactions, but most of these are resolved without reciprocal recombination by an RPA-BLM-containing complex; only those to which MLH1 is recruited may undergo reciprocal genetic exchange.

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The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination

Peter B. Moens, Nadine K. Kolas, Madalena Tarsounas, Edyta Marcon, Paula E. Cohen, and Barbara Spyropoulos
JCS 2002 115: 1611-1622. [Abstract] [Full Text]  

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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Search for Related Content