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Fig. 3. Dynein-GFP colocalizes with microtubules. Immunofluorescence staining of
IC-GFP-expressing cells with an anti-tubulin antibody followed by
Lissamine-conjugated secondary antibody. (a) Dynein-GFP signal. (b) Tubulin
staining. (c) Overlay of (a) and (b), with GFP in green and tubulin staining
in red. Yellow indicates colocalization. The arrowhead points to an example of
punctate dynein-GFP signals lining along a microtubule. The arrow points to an
example of dynein-GFP signal associated with microtubule plus end.