Fig. 6. Reporter analysis reveals feh-1 expression in the nervous system and in the neuromuscular structures of the pharynx. (A-C) show the expression pattern of a feh-1::GFP reporter fusion in living worms. The GFP gene from vector pPD95.75 fused to the 5' end of feh-1 is strongly expressed in neurons of the ventral cord and in the head, at the level of the pharynx, in a larva (A) and in an adult hermaphrodites (B,C). Arrowheads in (C) indicate some strongly expressing neuronal bodies, whereas the arrows show the neurites. The left panels show Nomarski images of the worms. Anterior is to the left, ventral is to the top of the figures. Bars, 100 µm. (D-G) shows the expression pattern of a 5'-feh-1::NLS::lacZ reporter fusion in fixed worms. lacZ gene from vector pPD21.28 fused to the 5' end of feh-1 produces a nuclear-targeted fusion protein, which allows us to detect the nuclei of the cells expressing the reporter. (D,E) show the heads of two worms, in which the staining of extra-pharyngeal neurons (D), and of pharyngeal cells (E) is evident, and is indicated as follows: white arrowheads, m3 cells; black arrowheads, m4 cells; arrows, m5 cells. The worm in (F) shows a detail of the central region of the body: the nucleus of a ventral chord neuron (black arrowhead), and a faint staining of cells close to the vulva (white arrowheads) is also seen. In (G), two tail neuronal nuclei are intensely stained. Bars, 20 µm.

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