Fig. 5. Pharyngeal localisation of FEH-1 by whole-mount immunofluorescence analysis. Anterior is to the left. Bar, 20 µm. (A) Immunodetection analysis of FEH-1 expression in adult hermaphrodite. Whole worms were fixed and stained with FEH-1 purified antibodies, followed by Texas-Red conjugated anti-rabbit antibody. (B) Worms were stained with FEH-1 and MH27 antibodies, followed by FITC-conjugated anti-rabbit antibodies to detect FEH-1 (green) and the Texas-Red conjugated anti-mouse antibody to detect JAM-1, the epithelial antigen recognised by MH27 (red). Samples were analysed by confocal microscopy. The merged image, at the bottom, shows no overlapping localisation of the two proteins. (C) Worms were stained with FEH-1-purified antibodies, followed by FITC-conjugated anti-rabbit antibodies (green); pharyngeal muscle was stained with Texas-Red-conjugated phalloidin (red). Samples were analysed by confocal microscopy. The merged image (yellow) at the bottom shows that FEH-1 is present both in muscle cells and outside.

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