Fig. 3. gb561 is a feh-1-null allele. (A) shows the structures of the predicted Y54F10AM.2 coding region (adapted from Wormbase) and of the actual feh-1 gene. The numbered bar at the top shows approximate sizes, in base pairs (bp). The exons are represented as filled boxes (black boxes, translated region; grey boxes, untraslated regions); roman numerals above the exons indicate their relative number. The yk423e6 sequence starts at the 3' end of exon 1. The ATG codons in exons 1 and 2 are also shown. The black bars below the feh-1 structure indicate the double deletion of the mutagenised allele gb561, whose structure is shown at the bottom. (B) Western blot analysis of protein lysates from +/+ (lane 1), +/gb561 (lane 2) and gb561/gb561 (lane 3) worms. Worms from the three populations were transferred into SDS buffer for protein extraction and western blot analysis with FEH-1 antibody. (C) Western blot analysis of protein lysates from untreated, mixed-stage Bristol N2 worms (lane 1) or from the F1 of feh-1 dsRNA-injected worms (lane 2). Harvested worms were lysed in SDS buffer and subjected to western blot with the FEH-1 antibody.

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