Fig. 7. PPH-4.1 localizes to centrosomes during mitosis. (A) Western blotting against a wild-type worm extract. Lane 1: detection by PPH-4.1 antibodies. Arrows indicate doublet bands corresponding to the predicted molecular mass of PPH-4.1 (37 kDa). Lane 2: detection by the antibodies pretreated with 10xHis-PPH-4.1 protein. (B) Localization of PPH-4.1 during early cleavages. (a,d,g,j,m,p) Anti--tubulin antibody staining. (b,e,h,k,n,q) Anti-PPH-4.1 antibody staining. (c,f,i,l,o,r) Merged images. (a-o) Wild-type embryos. PPH-4.1 was localized to centrosomes from prophase to anaphase and only weakly during telophase (a-1; PPH-4.1 staining on centrosomes is indicated by white arrows). During interphase, centrosomal localization of PPH-4.1 was not observed (m-o; interphase cells are indicated with white arrowheads). (p-r) pph-4.1(RNAi) embryo at prophase. No signal was detected in severely affected pph-4.1(RNAi) embryos. Bar, 10 µm.

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