Fig. 5. PPH-4.1 is required for aster formation and recruitment of -tubulin to centrosomes during mitosis and sperm meiosis. (A,F,K,N) DAPI staining showing chromosomes. (B,G,I,O) Anti--tubulin antibody staining. (C,H,M,P) Anti--tubulin antibody staining. (A-C) Wild-type embryo at pronuclear meeting. (F-H) pph-4.1(RNAi) embryo at pronuclear meeting. (D,E,I,J) Magnified view of the indicated area in B, C, G and H, respectively. (K-M) Wild-type primary spermatocyte showing a meiotic spindle. (N-P) pph-4.1(RNAi) primary spermatocyte showing condensed chromosomes similar to those in (K) and a spindle not properly organized. Note that astral microtubules were poorly organized from the centrosomes (G,I,O) and that -tubulin on the centrosomes was dispersed (H,J,P). Each embryo is aligned with the anterior end to the left. White arrowheads indicate centrosomal localization of -tubulin. Bar, 10 µm (A-C, F-H); 2 µm (D,E,I,J); 5 µm (K-P).

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