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Fig. 3. PPH-4.1 is required for proper chromosome segregation during sperm meiosis. Hoechst staining (blue) is overlaid on the Nomarski image. (A) Wild-type spermatids. Each spermatid contains one nucleus. (B) pph-4.1(RNAi) spermatids. White arrowheads indicate anucleated spermatids. Black arrowheads indicate binucleated spermatids. (C-F) Secondary spermatocytes with spermatids (arrowheads) budding from the residual body (arrows). (C,E) Wild-type. (D,F) pph-4.1(RNAi). Chromosomes are not properly segregated in pph-4.1(RNAi) spermatocytes. Bar, 10 µm (A,B); 5 µm (C-F).





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