Fig. 8. The spectrum of cellular phenotypes induced by p120ctn isoforms. P120ctn isoform 1A, expressed in 1205-Lu cells, fills up the entire cell volume, but less dense staining is evident in the cell nucleus (A) and in the lamellipodial extensions (a in panels A,B). Some cells demonstrate flattening and extensive lamellipodial protrusions (B,C). Thin filopodia (b in panels B,C) emerge from the cell body. The extending filopodia form branches (c in panel C). In the fully developed branching phenotype (D), dendritic extensions send out secondary branches (c in panel D). 1205-Lu cells grown in the chamber slides were transiently transfected with p120ctn isoforms and deletion constructs in parallel. Slides were processed for indirect immunofluorescence 16 hours later. Cells positive for the FLAG-tag-specific antibody M2 in each 0.7 cm² well were counted and divided into four categories (A-D) on the basis of their appearance. The percentage of cells in each category is shown (E). The total number of cells assessed in each well varied from 150 to 350. Bar, 50 µm.

Return to article