Fig. 4. Expression constructs encoding p120ctn isoforms, mutants and domains. Organization of p120ctn (A). Four alternative translation initiation sites within the N-terminal globular domain are shown as M1 to M4. The central part of p120ctn contains 10 Armadillo repeats. Two alternatively spliced exons, A and B, reside within the C-terminal domain. (B) Expression constructs encoding the p120ctn isoforms, deletions and domains, attached to the N-terminal FLAG-tag, were cloned into pcDNA3-vector. Proteins expressed by 1205-Lu cells, transiently transfected with each expression construct, were separated on a 9% PAGE, and the MAb M2 was used to demonstrate the FLAG-tagged proteins on the western blot (C). MW, molecular mass markers.

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