Fig. 2. Western blot analysis of p120ctn isoforms in normal and malignant epithelial cells. Cell lysates of neonatal human keratinocytes (NHK), HaCaT cells, squamous cell carcinomas (A431, A253, FADU, SCC12, DET562) (A) and normal and malignant melanoma cells (FM516SV3/3, WM793, 1205-LU, WM164, 451-LU) (B) were resolved on 6% PAGE and proteins detected with the MAb pp120 (A, and lower panel in B) and with MAb 6H11 (B, upper panel). The position of the bands corresponding to p120ctn isoforms 1 to 4 are shown on the right side of the blot together with the positions of molecular weight markers. Note that the isoform 1 is specific for melanocytic cells, whereas normal keratinocytes express predominantly isoform 3. A slower migrating band either corresponding to isoform 2 or to the phosphorylated form of isoform 3 is prominent, especially in squamous carcinoma cells. Isoform 4 is present in normal keratinocytes but is largely lost from the tumor cells.

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