Fig. 1. Implantation of fluorescence-labelled cultured mesothelial cells and peritoneal lavage-derived cells on representative areas of serosal injury. DiI-labelled cultured mesothelial cells, characterised by intense, punctate, red fluorescence in cell suspension (inset A), were present on imprints of regenerating mesothelium 5 days post injury (A), whereas DiI-labelled cultured fibroblasts were absent at the same time point (B). DiI-labelled peritoneal lavage cells were also present 8 days post injury (C). DiI-labelled cells (arrowed) were clearly distinguished from adjacent unlabelled cells (arrowheads). PKH26-PCL-labelled phagocytic cells were characterised by intense cytoplasmic red fluorescence in peritoneal lavage fluid obtained 2 days after peritoneal abrasion injury (inset D). Labelled macrophages (arrowed) were implanted onto the wound surface at 3 and 5 days post injury (D and E, respectively) but were absent by 8 days (F). Bars, 10 µm.

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