Fig. 10. F-actin is required for normal cytokeratin assembly and organization in vivo. Confocal, single label micrographs showing cytokeratin distribution in Xenopus eggs. In the absence of activation, both control (A) and latrunculin-treated (C) eggs have no apparent cytokeratin network, although cytokeratin aggregates not seen in controls were seen in latrunculin-treated, unactivated eggs. Following activation, cytokeratin assembles into a fine network in control eggs (B). Cytokeratin in activated, latrunculin-treated eggs ranges from moderately disordered, forming unusually thick cables and loops (D), to completely disordered, forming extremely large aggregates (E). F is a bar graph displaying for two experiments the difference in width of filaments and/or aggregates in control versus latrunculin-treated, activated eggs. Latrunculin treatment resulted in increased thickness, as well as overall variability. The error bars for the width of filaments in control, activated eggs were too small to appear on this graph (P<0.05).

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