Fig. 6. The lateral diffusional mobility of MAN1 is decreased in the nuclear envelope compared with the ER. Confocal fluorescence studies of localization and mobilities of the MAN1-GFP fusion protein encoded by plasmid MAN538-GFP (see legend to Fig. 3) measured by qualitative FRAP, showing photobleach recovery in (A) nuclear envelope membranes and in (B) ER membranes. The fluorescence in the boxed regions was bleached and the fluorescence recovery is shown at 10, 60 and 290 seconds after the bleaching. Bar, 5 µm.

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