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Interphase chromosome arrangements

Chromosomes in most interphase nuclei have a specific non-random distribution. Centromeres lie near one pole, whereas telomeres point towards the other (the `Rabl orientation'). Moreover, chromosomal regions are positioned in specific subnuclear domains, which could have an important role in gene regulation. One system used for analysis of chromosome distribution is the tetO/TetR-GFP system, in which binding of a transgenic TetR-GFP fusion protein to integrated copies of its target sequence (tetO) highlights chromosomal regions containing these sequences. Josef Loidl and co-workers now show that integration of tetO alters the architecture of the budding yeast nucleus (see p. 1213): it causes tetO-containing chromosomal regions to associate, which can perturb the normal Rabl orientation. Interestingly, the association of tetO sequences is not an inherent property but requires TetR molecules. The authors therefore conclude that TetR physically connects tetO sequences from different loci. The tetO/TetR-GFP system might thus represent a useful model for protein-mediated regulation of interphase chromosome organization.

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