Fig. 6. Contraction of FN fibrils following cytochalasin B treatment. Images were taken before and 10 and 20 minutes after adding 4 µM cytochalasin B. Actin filament bundles persisted at 10 minutes but collapsed into irregular islands at 20 minutes. The FN fibril indicated by the arrow was extended from an attachment point on the pseudopod up to the cell body at both 0 and 10 minutes. When the actin filament bundles disappeared at 20 minutes, this fibril contracted towards the bottom attachment point. That bottom attachment point also changed from a curved FN fibril to a straight one at 20 minutes, as other attachment points were lost. The bottom two panels show a superimposition of the FN image only at 0-10 and 10-20 minutes. Bar, 20 µm.

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