Fig. 5. An area of cell culture (after 72 hours of culture) showing some mature FN matrix fibrils and several areas where matrix fibril assembly is being initiated. The cfp-Moe staining of the actin cytoskeleton is shown in red, and the FN-yfp in green. The cfp-Moe expression is variable: it is high for the cell in the center, medium for the one at the top and low for the cell at the bottom right. There is a cell at the middle right that is not expressing cfp-Moe at all (its nucleus is indicated by a dotted line at 0:00 and is faintly visible at 1:00). Five sites of matrix assembly are identified by numbers at 0:00. A movie is available in supplementary results, showing these and intervening frames, carefully aligned to fixed points on the substrate. The most informative view of matrix assembly is obtained by stepping back and forth between frames of the movie. Bar, 50 µm.

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