Fig. 1. Maps of tetO integration sites and FISH probes on chromosome V. (A) One tetO tract is integrated 35 kb away from the centromere on the short arm at the URA3 locus. The other integration site is 30 kb away from the right telomere at the BMH1 locus. (B) Yeast strains with different tetO inserts used in the experiments. Diploid strains are homozygous for CEN tetO or TEL tetO or heterozygous for the two inserts on chromosomes V. A haploid strain has both inserts in the same chromosome (cis). These strain constructs exist both as versions expressing and not expressing TetR-GFP (grey ovals, centromeres; green rectangles, integrated tetO sites in the presence of TetR; grey rectangles, integrated tetO sites in the absence of TetR). (C) Map of FISH probes for regions adjacent to centromeric (1), telomeric (2) tetO insertion sites, and for the tetO sequence (3).

Return to article