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Fig. 4. Expression of PPAR{gamma} and adipsin transcripts in 3T3-L1 transfectants. Total RNA isolated on days 0, 1, 2, 3, 5 and 7 after adipogenic induction was analyzed by semi-quantitative RT-PCR as described in Materials and Methods. Results are representative of five independent experiments on three individual clones of each transfectant type. The levels of PPAR{gamma} and adipsin transcripts markedly increased in control cells during differentiation. In {alpha}1-PDX cells, the increase was significantly lower for adipsin and not observed for PPAR{gamma}.





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