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Fig. 2. 
1-PDX expression inhibits 3T3-L1 adipocyte differentiation. (A)
Three control and 16 1-PDX 3T3-L1 independent transfectant cell lines
at confluence were treated (or not) with adipogenesis-inducing agents for 2
days. They were subsequently cultured for 5 days, fixed and stained with Oil
Red O on day 7. A typical staining of differentiated and undifferentiated
cells is shown. Unlike control cells, 1-PDX transfectant cell lines
failed to respond to the adipogenic treatment. (B) Expression of 1-PDX
transgene by RT-PCR in control and 1-PDX transfectants cell lines.
Transcripts were observed in 1-PDX cells and not in control cells.
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